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Protective effect of tomatine against hydrogen peroxide-induced neurotoxicity in neuroblastoma (SH-SY5Y) cells
作者:kyb 点击数:时间:2014-05-14 09:29:34

Protective effect of tomatine against hydrogen peroxide-induced neurotoxicity in neuroblastoma (SH-SY5Y) cells[1]

Shao-lan Huang, Hai-bo He *, Kun Zou *, Cai-hong Bai, Yan-hong Xue, Jun-zhi Wang, Jian-feng Chen

Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Science, China Three Gorges University, Yichang, China

Abstract

Objectives Reactive oxygen species (ROS) can induce cell apoptosis, and oxidative stress has been implicated in a variety of neurodegenerative disorders. Tomatine, which is a naturally occurring steroidal glycoalkaloid isolated from Solanum cathayanum, has shown potent antioxidant properties.

Methods In the present study, we used the SH-SY5Y cell line as an in vitro model, and investigated the protective effect of tomatine against hydrogen peroxide (H2O2)-induced neurotoxicity in SH-SY5Y cells.

Key findings Tomatine might inhibit the release of cellular lactate dehydrogenase, increase antioxidant enzyme activities and glutathione content, reverse the downregulated protein expression of the brain-derived neurotrophic factor (BDNF), inhibit expression of Bax and activations of caspase-3 and caspase-9 in H2O2-induced SH-SY5Y cells.

Conclusions Tomatine exerted beneficially neuroprotective effect on H2O2-induced SH-SY5Y cells, mainly enhancing intracellular antioxidant enzyme activities and BDNF expression, inhibiting H2O2-induced oxidative stress as well as expression of Bax and activations of caspase-3 and caspase-9, alleviating H2O2-induced SH-SY5Y cell injury and cell death.



Correspondence to: Hai-bo He and Kun Zou. Key Laboratory of Natural Products Research and Development, China Three Gorges University, 8, University Avenue, Yichang, Hubei, China. E-mail: haibohe2001@gmail.com and kzou@ctgu.edu.cn.

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